Crosslinking Reagents
Thermo Scientific™ BM(PEG)2 (1,8-bismaleimido-diethyleneglycol)
Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, pegylated, water-soluble crosslinker composed of maleimide groups and 2-unit (14.7A) PEG spacer arm.
Thermo Scientific™ Sulfo-SDA (Sulfo-NHS-Diazirine) (sulfosuccinimidyl 4,4'-azipentanoate)
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).
Thermo Scientific™ NHS-PEG4-Azide
Activated azido compounds to derivatize proteins or amine surfaces for selective conjugation, detection or purification by phosphine-modified probes.
Thermo Scientific™ SM(PEG)24 (PEGylated, long-chain SMCC crosslinker)
Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).
Thermo Scientific™ BS3-d0 (bis(sulfosuccinimidyl) suberate-d0)
Crosslink amines to amines using these heavy (deuterated) and light matched pairs of an 11.4A, NHS-ester crosslinker for mass spectrometry analysis of protein interactions.
Thermo Scientific™ SM(PEG)8 (PEGylated, long-chain SMCC crosslinker)
Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).
Thermo Scientific™ BS(PEG)9
Crosslink amines to amines with these long (21.7A and 35.8A) crosslinkers composed of NHS-ester groups and 5-unit or 9-unit PEG spacer arms that enhance protein solubility.
Thermo Scientific™ BM(PEG)3 (1,11-bismaleimido-triethyleneglycol)
Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, pegylated, water-soluble crosslinker composed of maleimide groups and 3-unit (17.8A) PEG spacer arm.
Thermo Scientific™ SDAD (NHS-SS-Diazirine) (succinimidyl 2-((4,4'-azipentanamido)ethyl)-1,3'-dithiopropionate)
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).
Thermo Scientific™ BS3-d4 (bis(sulfosuccinimidyl) 2,2,7,7-suberate-d4)
Crosslink amines to amines using these heavy (deuterated) and light matched pairs of an 11.4A, NHS-ester crosslinker for mass spectrometry analysis of protein interactions.
Thermo Scientific™ SIA (succinimidyl iodoacetate)
Crosslink amines to sulfhydryls with this very short crosslinker composed of NHS-ester and iodoacetyl (haloacetyl) groups at ends of a 3-atom (1.5A) spacer arm.
Thermo Scientific™ EZ-Link™ TFP Ester-PEG12-DBCO
A heterobifunctional crosslinker that contains an amine reactive tetrafluorophenyl (TFP) and a dibenzylcyclooctyne (DBCO) with a water solubilizing long PEG12 spacer in between. Use to label any primary amine containing molecule such as a protein to biomolecule with a distantly attached DBCO group.
Thermo Scientific™ NHS-Phosphine
Derivatize proteins or amine surfaces for selective conjugation, detection or purification of azide-tagged targets with these activated phosphine compounds.
Thermo Scientific™ Pierce™ Premium Grade Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)
Optimize applications where product integrity and risk minimization are paramount our highest quality formulation of this popular amine-to-sulfhydryl crosslinker.
