Crosslinking Reagents
Thermo Scientific™ SM(PEG)6 (PEGylated, long-chain SMCC crosslinker)
Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).
Thermo Scientific™ BM(PEG)3 (1,11-bismaleimido-triethyleneglycol)
Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, pegylated, water-soluble crosslinker composed of maleimide groups and 3-unit (17.8A) PEG spacer arm.
Thermo Scientific™ BM(PEG)2 (1,8-bismaleimido-diethyleneglycol)
Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, pegylated, water-soluble crosslinker composed of maleimide groups and 2-unit (14.7A) PEG spacer arm.
Thermo Scientific™ Mass Spectrometry Compatible Crosslinkers
Improve mass spectrometry detection of crosslinked proteins and interactions with DSPP and TBDSPP heterotrifunctional crosslinkers.
Thermo Scientific™ EZ-Link™ Maleimide-PEG4-DBCO
A sulfhydryl reactive heterobifunctional crosslinker which contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry applications. The thiol/sulfhdryl reactive maleimide can be used to label a variety of thiol containing moieties such as free cystines in proteins, peptides, or other (bio)molecules.
Thermo Scientific™ EZ-Link™ TFP Ester-PEG4-DBCO
An amine reactive heterobifunctional crosslinker which contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry applications. The crosslinker can be used to label a variety of amine containing molecules such as proteins, biomolecules, or small molecules.
Thermo Scientific Pierce™ Pierce™ SM(PEG)12, No-Weigh™ Format
Thermo Scientific™ Pierce™ SM(PEG)12 is hetero-bifunctional crosslinker with N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules.
Thermo Scientific™ BS3-d0 (bis(sulfosuccinimidyl) suberate-d0)
Crosslink amines to amines using these heavy (deuterated) and light matched pairs of an 11.4A, NHS-ester crosslinker for mass spectrometry analysis of protein interactions.
Thermo Scientific™ SIA (succinimidyl iodoacetate)
Crosslink amines to sulfhydryls with this very short crosslinker composed of NHS-ester and iodoacetyl (haloacetyl) groups at ends of a 3-atom (1.5A) spacer arm.
Thermo Scientific™ SM(PEG)24 (PEGylated, long-chain SMCC crosslinker)
Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).
Thermo Scientific™ SDAD (NHS-SS-Diazirine) (succinimidyl 2-((4,4'-azipentanamido)ethyl)-1,3'-dithiopropionate)
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).
Thermo Scientific™ L-Photo-Methionine
Diazirine analogs of leucine and methionine to express proteins in cell culture that will crosslink their protein interactors upon UV-light activation in vivo.
Thermo Scientific™ Sulfo-EMCS (N-ε-maleimidocaproyl-oxysulfosuccinimide ester)
Crosslink amines to sulfhydryls with this mid-length crosslinker composed of sulfo-NHS-ester and maleimide groups at ends of a water-soluble, 8-atom (9.4A) spacer arm.
Thermo Scientific™ Pierce™ Premium Grade Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)
Optimize applications where product integrity and risk minimization are paramount our highest quality formulation of this popular amine-to-sulfhydryl crosslinker.
Thermo Scientific™ Pierce™ Premium Grade DSP (dithiobis(succinimidyl propionate))
Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this popular amine-reactive, cleavable crosslinker.